제어 방출형 근관 소독제로부터 클로르헥시딘의 방출에 미치는 3가지 POLYMER의 제어효과
The sustaining effect of three polymers on the release of chlorhexidine from a controlled release drug device for root canal disinfection
소속 상세정보
복영빈/Bok YB
이덕연/이찬영/김경남/금기연/Lee DY/Lee CY/Kim KN/Kum KY
KMID : 0362320040290060548
Abstract
본 연구는 서방형 근관소독제(CRD)의 prototype으로 부터 chlorhexidine (CHX) 의 방출 속도를 제어하기 위한 3가지 polymer (chitosan, PMMA, PLGA) 의 제어 효과를 평가하기 위해 이루어졌다. 80번 paper point (Sure-Endo TM, #80)에 20% CHX를 loading 한 후 각 군당 10 개씩 4 군으로 분류하였다; Croup A: Non-polymer coated prototype, Group B: chitosan-coated prototype, Group C: PMMA-coated prototype, Group D: PLGA-coated prototype. 각각의 paper point에 함유된 CHX양을 동일하게 하기 위해, CHX loading후 무게를 측정하여 유사한 무게의 sample을 선택하여 사용하였다. 모든 시편은 3ml증류수가 담긴 큐벳에 넣은 후 3, 6, 10, 20, 30,40, 50분 마다. 1, 2, 3,4, 5, 6시간 마다 각각 10㎕씩 채취하고, 1주일 후 다시 10㎕을 채취하여 UV흡광도를 이용하여 CHX의 방출 속도를 비교하였다. 또한, 각 prototype의 표면 관찰을 위하여 100배와 5000배의 주사전자 현미경을 이용하여 표면 구조를 촬영하였다. 1. CHX 의 방출 속도는 non-coated groupo, chitosan-coated group, PLGA-coated grouop, PMMA-coated group순이었으며 각 군간에는 통계학적 인 유의차가 있었다 (p < 0.05). 2. PMMA 나 PLGA 를 도포한 CRD 표면에서만 pore가 관찰되었으며 pore size가 커질수록 방출 속도가 빨랐다. 결론적으로 polymer coating에 의해 제어방출형 근관소독제의 protoytype 으로 부터 약물 (CHX)의 방출 속도를 제어 할수 있었다.
The aim of this in vitro study was to evaluate the suitability of using chitosan, poly (lactide-co-glycolide) (PLGA), and polymethyl methacrylate (PMMA) to control the release of chlorhexidine diglucon ate (CHX) from a prototype of controlled release drug device (CRD) for root canal disinfection. Four different prototypes with different formulations were prepared. Group A (n = 12): The device (absorbent paper point) was loaded with CHX as control. Group B (n = 12): same as group A. but the device was coated with chitosan. In Groups C and D, the device was treated in the same way as group A and then coated three times with 5% PMMA (Group C, n = 12), or coated three times with 3% PLGA (Group D, n = 12). The devices were randomly allocated to experimental groups of 12 each. All CRD prototypes were soaked in 3 mL distilled water. The concentrations of CHX were determined using a UV spectrophotometer. The surface characteristics of each prototype were observed using a scanning electron microscope. The result showed that release rate of CHX was the greatest in the non-coated group, followed by the chitosan-coated group, the PLGA-coated group, and the PMMA-coated group (P < 0.05). Pores were observed on the surface of the prototypes that were coated with PLGA and PMMA. When the pore size was smaller, the release rate was lower. This data indicate that polymer coating can control the release rate of CHX from the CRD prototypes.
키워드
제어방출형 근관소독제;클로르헥시딘;Controlled release drug device;Chlorhexidine digluconate;Chitosan;Polymethyl methacrylate;Poly (lactide-co-glycolide);Root canal disinfectant
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